Sampling and pre-detection of infectious diseases are effective ways to prevent them from widely spreading. Among the conventionally used sampling methods, the cyclone-based air sampler is considered the most effective and simplest method. In this paper, we rebuilt a new cyclone-type air sampler device by modifying the size and shape of a commercially available product, Coriolis µ (Bertin Technology). This newly built air sampler’s collection efficiency was measured using polystyrene particles by comparing the amount before and after sampling. Additionally, for the feasibility of sampling of airborne virus, recombinant influenza virus antigen (H1N1) was injected into the custom-built air chamber where the air sampler’s inlet was connected so that the antigen was collected through sampling process. The solution was measured using a custom-made electrochemical platform which consists of antibody conjugated Au-electrode on PCB and small size reading system. Our findings showed that the influenza antigen was collected with good efficiency as well as detected with high sensitivity.