The stable isotope of carbon, 13C, has been used in several studies for source characterization of carbonaceous aerosol since there are specific signatures for different sources. In rural areas, the influence of different sources is complex and the application of δ13C for source characterization of the total carbonaceous aerosol (TC) can therefore be difficult, especially the separation between biomass burning and biogenic sources. We measured δ13C from 25 filter samples collected during one year at a rural background site in southern Sweden. Throughout the year, the measured δ13C showed low variability (–26.73 to –25.64‰). We found that the measured δ13C did not correlate with other commonly used source apportionment tracers (14C, levoglucosan). δ13C values showed lower variability during the cold months compared to the summer, and this narrowing of the δ13C values together with elevated levoglucosan concentrations may indicate contribution from sources with lower δ13C variation, such as biomass or fossil fuel combustion. Comparison of two Monte Carlo based source apportionment models showed no significant difference in results when δ13C was incorporated in the model. The insignificant change of redistributed fraction of carbon between the sources was mainly a consequence of relatively narrow range of δ13C values and was complicated by an unaccounted kinetic isotopic effect and overlapping δ13C end-member values for biomass burning and biogenic sources.