In this research, high performance nanofiber filters were developed by improving nanofiber deposition uniformity on substrate media as well as manufacturing branched nanofibers with additional nanofibers spun out from the main nanofibers. Their filtration performance was compared with regular nanofiber filters and beaded nanofiber filters in terms of particle removal efficiency, filter pressure drop and particle loading capacity. First, nanofiber layer deposition uniformity was improved by removing substrate surface charge during the electrospinning process. The nanofiber deposition uniformity was visually evaluated under an optical microscope and an electron microscope. Also, the uniform nanofiber filters exhibited higher filtration efficiency with lower filter pressure drop when compared with nonuniform nanofiber filters manufactured by the conventional electrospinning setup. Second, branched nanofiber media, which has numerous additional nanofibers spun out from the main nanofibers, was manufactured by controlling polymer solution viscosity. The filtration performance of nanofiber filters was improved dramatically by decreasing airflow resistance with (1) longer streamwise inter-fiber distance, (2) smaller nanofiber diameter. As well, the filtration efficiency increased by (3) additional nanofibers spun out from the main nanofibers.