Recent studies have shown that air pollution is intense and hazardous in Asia compared to other parts of the world due to late and poor implementation of updated technology in automobiles and industry, as well as high population density. Respiratory diseases, including asthma, are exacerbated by air pollution. However, effects of PM2.5 especially in Asian cities have not yet been well studied, notably on exacerbation of respiratory allergy in this continent. In this study, airway epithelial cells were exposed to crude PM2.5 particle collected by cyclone technique from three different Asian cities, namely Sakai, Bangkok, and Taipei. We compared the cytotoxicity and inflammatory potential of PM2.5 among the three cities by measuring IL-6 and IL-8 release. Samples from Sakai and Bangkok showed cytotoxic effects at a dose of 75 µg ml-1. Moreover, PM2.5 collected from Sakai and Bangkok induced IL-6 and IL-8 release even at low doses. IL-6 and IL-8 release was highly associated with fluoranthene derivatives, microbial factors (endotoxin and β-glucan), metals (Ti), and organic carbon (OC2, OC3) and elemental carbon (EC1) in PM2.5. These components of PM2.5 collected from Asian cities can contribute to cellular damage and pro-inflammatory responses in airway epithelial cells, and the effect depends on PM2.5 sources in the locations.