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Volume 14, No. 6, October 2014, Pages 1714-1725 PDF(1.18 MB)  
doi: 10.4209/aaqr.2013.07.0260   

Metabolomic Analysis of the Effects of Motorcycle Exhaust on Rat Testes and Liver

Ching Yu Lin1,2,3,4, Shang-Ying Wu1, Hao-Jan Liang1, Yi-Chun Liu2, Tzuu-Huei Ueng2

1 Institute of Environmental Health, College of Public Health, National Taiwan University, No. 17, Xu-zhou Rd., Taipei 100, Taiwan
2 Graduate Institute of Toxicology, College of Medicine, National Taiwan University, No. 1, Sec. 1, Jen-ai Rd., Taipei 100, Taiwan
3 Center for Environment and Occupational Health Research, College of Public Health, National Taiwan University, No. 17, Xu-zhou Rd., Taipei 100, Taiwan
4 Research Center for Genes, Environment and Human Health, College of Public Health, National Taiwan University, No. 17, Xu-zhou Rd., Taipei 100, Taiwan

 

Highlights
  • Motorcycle exhaust (ME)-induced male reproductive toxicity was examined in rats.
  • The molecular mechanisms of ME were examined using metabolomic approach.
  • The testicular toxicity is related with lipid- and energy-related metabolism.
  • Treatment with vitamin E can partially reverse the molecular events in the testes.

Abstract

 

Motorcycles are one of the primary means of transportation in many Asian metropolitan areas. Exposure to motorcycle exhaust (ME) has been shown to cause male reproductive toxicity in a rat inhalation model. In the present study, the molecular mechanisms underlying ME-induced male reproductive toxicity were examined in a rat model using nuclear magnetic resonance (NMR)-based metabolomics. Rats were treated with 1:10-diluted ME for 1 hour in the morning and 1 hour in the afternoon, daily, via head-nose-only exposure chambers, for a period of four weeks. Half of the ME-exposed rats were co-treated with vitamin E to examine its protective effects. Both hydrophilic and hydrophobic metabolites from the testes and liver were extracted for 1H and J-resolved NMR analyses. The obtained NMR spectra were converted into series of spectral binned areas and subjected to orthogonal projections to latent structure-discriminant analyses. Decreased spermatid counts and elevated levels of betaine in the testes of rats in the ME-treated group were partially attenuated by vitamin E co-treatment. Although it was not statistically significant, we observed a consistent trend of partial reversal of the elevated levels of alanine, glycine, leucine, tyrosine, and valine in the testes of rats in the ME-treated group due to vitamin E co-treatment. Decreases in testicular ethanolamine and inositol were only significant in the group co-treated with ME and vitamin E. The testicular toxicity of ME may be due to alterations in lipid- and energy-related metabolism. We conclude that ME generates greater metabolic responses in the testes than in the liver. Treatment with vitamin E can partially reverse the molecular events in the testes.

 

 

Keywords: Motorcycle exhaust; Metabolomics; Male reproductive toxicity; Nuclear magnetic resonance; Testes.

 

 

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